✍ Scribed by I. Molnár-Perl; V. Fábián-Vonsik
No coin nor oath required. For personal study only.
Earlier proposals aimed at increasing the detector responses of histidine in gas chromatographic analysis have been compared and contrasted with regard to their effect on the other amino acids present. Twenty-one amino acids have been measured in the form of their N,O,(S)-trifluoroacetyl (TFA) isobutyl esters. Detector responses of the amino acid N,O,(S)-TFA isobutyl esters of dichloromethane solutions alone and mixed with acetic anhydride were recorded and compared with those of other TFA esters. Reproducibility data for chosen representative amino acid N,O,(S)-TFA isobutyl esters are presented.
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Previous studies in our laboratory (1,2) have shown that the phenylt,hiohydantoin (PTH) derivative of all the amino acids except arginine could be analyzed in submicrogram amounts by gas chromatography. The analysis was performed by direct injection of all derivatives except the aspartyl, glutanlyl,
A practical method for the quantitative determination of protein amino acids by gas-liquid chromatography (GLC) is described. All of the common protein amino acids except arginine can be readily converted into their N-isobutyloxycarbonyl (N-isoBOC) methyl ester derivatives by a simple procedure invo
The effect of tryptophan on the biosynthesis of proline has been investigated. Cells of Daucus carota grown in B5 medium supplemented with 5×10(-4)M tryptophan acquired the ability to grow in the presence of inhibitory concentrations of azetidine-2-carboxylic acid, an analog of proline. When trp was