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Effect of exogenous amino acids on the intracellular content of proline and other amino acids inDaucus carotacells

✍ Scribed by Daniela Carbonera; Paolo Iadarola; Rino Cella

Publisher
Springer
Year
1989
Tongue
English
Weight
285 KB
Volume
8
Category
Article
ISSN
0721-7714

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✦ Synopsis

The effect of tryptophan on the biosynthesis of proline has been investigated. Cells of Daucus carota grown in B5 medium supplemented with 5Γ—10(-4)M tryptophan acquired the ability to grow in the presence of inhibitory concentrations of azetidine-2-carboxylic acid, an analog of proline. When trp was added to carrot cell cultures at sub-growth inhibiting concentrations, overproduction of intracellular free proline was observed. An increase was also observed for lys, his, ala, leu and phe. Likewise, the addition of asparagine, glutamic acid and phenylalanine to the medium stimulated the intracellular increase of free proline and other amino acids.

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