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Gap-43 immunoreactivity and axon regeneration in retinal ganglion cells of the rat

✍ Scribed by Schaden, H. ;Stuermer, C. A. O. ;Bähr, M.

Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
854 KB
Volume
25
Category
Article
ISSN
0022-3034

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✦ Synopsis

Retinal ganglion cells (RGCs) in rats were retrogradely labeled with the fluorescent tracer Fluorogold (FG) and subjected to GAP-43 and c-JUN immunocytochemistry to identify those RGCs that are capable of regenerating an axon. After optic nerve section (ONS) and simultaneous application of FG to the nerve stump (group 1 experiments), GAP-43 immunoreactive RGCs (between 2 and 21 days after ONS) always represented a subfraction of both FG-labeled (i.e., surviving) RGCs and RGCs exhibiting c-JUN. GAP-43 immunoreactive RGCs represented 22% of RGCs normally present in rat retinae and 25% of surviving RGCs at 5 days after O N S but were reduced to 2% and 1%, which is 6% and 5% of survivors at 14 and 21 days, respectively. In animals that received a peripheral nerve ( P N ) graft after O N S (group 2 experiments), RGCs with regenerating axons were identified by FG application to the graft at 14 and 21 days. When

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