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Gangliosides stimulate astroglial cell proliferation in the absence of serum

✍ Scribed by Ritsuko Katoh-Semba; Laura Facci; Stephen D. Skaper; Silvio Varon

Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
700 KB
Volume
126
Category
Article
ISSN
0021-9541

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✦ Synopsis

Previous studies with microcultures of astroglial (AG) cells from newborn rat cerebrum had shown a n ability of gangliosides to interact with AG cells cultured under defined conditions. We have now investigated t h e capability of gangliosides to stimulate DNA synthesis and cell number increases in similar secondary microcultures of newborn rat cerebrum AG cells. At a concentration of 6 x 10-5M, GM1 ganglioside stimulated DNA synthesis and increased cell numbers, with DNA synthesis leading cell increases by 12-24 hr. The ganglioside-induced AG cell proliferative response occurred with GDla, CDlb and GTlb, GTlb being t h e most potent at 10-5M-while asialo GMl and sialic acid were without effect. In the standard test cultures, DNA synthesis declined very steeply after the first day, with cell numbers stabilizing at the level reached after 2 days. Ganglioside was not itself responsible for the restricted proliferative response, as serum produced the same behaviors.

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