✦ LIBER ✦

Ionic responses and growth stimulation in rat astroglial cells: Differential mechanisms of gangliosides and serum

✍ Scribed by Stephen D. Skaper; Silvio Varon

Publisher: John Wiley and Sons
Year: 1987
Tongue: English
Weight: 675 KB
Volume: 130
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041300320

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✦ Synopsis

Rat astroglial cells respond to fetal calf serum (FCS) and gangliosides, including GMI, by undergoing proliferation. Here, we show that addition of FCS but not GM1 causes an increase in Na+, K+-pump activity, as measured by ouabain-sensitive 86Rb+ influx. The increase of Na+, K+-pump activity by FCS was due to increased Na+ influx (measured with 22Na+). This increased Na+ influx was sensitive to amiloride, an inhibitor of Na+/H + exchange. Amiloride also blocked the FCS-stimulated incorporation of [3H]thymidine into DNA. Two defined polypeptide growth factors, epidermal growth factor and fibroblast growth factor were also able to elicit a n amiloride-sensitive Na+ influx and an ouabain-sensitive K + uptake in these astroglial cells, in the presence of FCS or insulin. Thus, GM1 differs from serum and growth factors in the mechanisms by which these agents stimulate t h e proliferation of the astroglial cells used here.

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