Ganglioside Modulation of Cyclic AMP-Dependent Protein Kinase and Cyclic Nucleotide Phosphodiesterase In Vitro
โ Scribed by Allan J. Yates; John D. Walters; Catherine L. Wood; David Johnson
- Book ID
- 111175125
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 640 KB
- Volume
- 53
- Category
- Article
- ISSN
- 0022-3042
No coin nor oath required. For personal study only.
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Limited proteolysis of catalytic and regulatory subunits of cyclic AMP-dependent protein kinase (A-pk), cyclic AMP phosphodiesterase, glycogen synthase, and histones by fungal protease (type XIX) was analyzed by the digested peptide bands in SDS polyacrylamide gel electrophoresis. The modulatory eff
Skin from hairless mice was incubated with two synthetic retinoids, isotretinoin and etretinate, and the cAMP content as well as the activity of cAMPdependent protein kinase were determined. A crude plasma membrane preparation was used to measure adenylyl cyclase activity. Neither isotretinoin (10 -