𝔖 Bobbio Scriptorium
✦   LIBER   ✦

G Protein Signaling Volume 237 || Analysis of G Protein-Mediated Activation of Phospholipase C in Cultured Cells

✍ Scribed by Smrcka, Alan V.

Book ID
121012876
Publisher
Humana Press
Year
2003
Tongue
English
Weight
84 KB
Category
Article
ISBN-13
9781592594306

No coin nor oath required. For personal study only.

✦ Synopsis

Alan V. Smrcka Presents A Collection Of Cutting-edge Methods For Investigating G Protein Signaling From A Variety Of Perspectives Ranging From In Vitro Biochemistry To Whole Animal Studies. Among The Readily Reproducible Techniques Presented Are Those For The Purification Of G Proteins And Effectors Enzymes, Assays Of These Purified G Proteins And Effector Enzymes, And For The Study Of G Proteins Interactions With Effectors In Intact Cells. Additional Methods Are Provided For Assaying G Protein Coupled Receptor Structure, Function, And Localization, And For Studying The Physiological Roles For Endogenous G Proteins.

πŸ“œ SIMILAR VOLUMES

Coupling of bradykinin receptors to phos
Coupling of bradykinin receptors to phospholipase C in cultured fibroblasts is mediated by a G-protein
✍ Beth G. Etscheid; Mitchel L. Villereal πŸ“‚ Article πŸ“… 1989 πŸ› John Wiley and Sons 🌐 English βš– 802 KB

In cultured foreskin fibroblasts, bradykinin stimulates inositol phosphate generation, arachidonic acid release, and N a + / H + exchange, with doses of 1-3 n M yielding half-maximal stimulation. Binding of 'H-bradykinin to these cells demonstrates a single receptor site with a Kd of 2.0 n M and a B

G Protein Signaling Volume 237 || Purifi
G Protein Signaling Volume 237 || Purification of G Protein Subunits from Sf9 Insect Cells Using Hexahistidine-Tagged Ξ± and Ξ²Ξ³ Subunits
✍ Smrcka, Alan V. πŸ“‚ Article πŸ“… 2003 πŸ› Humana Press 🌐 English βš– 243 KB

Alan V. Smrcka Presents A Collection Of Cutting-edge Methods For Investigating G Protein Signaling From A Variety Of Perspectives Ranging From In Vitro Biochemistry To Whole Animal Studies. Among The Readily Reproducible Techniques Presented Are Those For The Purification Of G Proteins And Effectors