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G Protein Signaling Volume 237 || Purification of G Protein Subunits from Sf9 Insect Cells Using Hexahistidine-Tagged α and βγ Subunits

✍ Scribed by Smrcka, Alan V.


Book ID
115513090
Publisher
Humana Press
Year
2003
Tongue
English
Weight
243 KB
Category
Article
ISBN-13
9781592594306

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✦ Synopsis


Alan V. Smrcka Presents A Collection Of Cutting-edge Methods For Investigating G Protein Signaling From A Variety Of Perspectives Ranging From In Vitro Biochemistry To Whole Animal Studies. Among The Readily Reproducible Techniques Presented Are Those For The Purification Of G Proteins And Effectors Enzymes, Assays Of These Purified G Proteins And Effector Enzymes, And For The Study Of G Proteins Interactions With Effectors In Intact Cells. Additional Methods Are Provided For Assaying G Protein Coupled Receptor Structure, Function, And Localization, And For Studying The Physiological Roles For Endogenous G Proteins.


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✍ Smrcka, Alan V. 📂 Article 📅 2003 🏛 Humana Press 🌐 English ⚖ 290 KB

Alan V. Smrcka Presents A Collection Of Cutting-edge Methods For Investigating G Protein Signaling From A Variety Of Perspectives Ranging From In Vitro Biochemistry To Whole Animal Studies. Among The Readily Reproducible Techniques Presented Are Those For The Purification Of G Proteins And Effectors