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Further characterization of a DNA polymerase activity in mouse sperm nuclei

โœ Scribed by M. Philippe; Ph. Chevaillier


Book ID
115746466
Publisher
Elsevier Science
Year
1976
Weight
972 KB
Volume
447
Category
Article
ISSN
0005-2787

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A nuclear DNA-polymerase activity has been detected in situ in mouse spermatozoa with a cytochemical method. The acid-insoluble radioactive product obtained after incubation in a mixture containing all four deoxyribonucleoside 5'-triphosphates, Mg++,KCl and dithiothreitol (DTT) was completely remove

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## Abstract DNA polymerase activity was measured in mouse embryos at stages before implantation to determine whether it increases in proportion to the amount of DNA synthesis, as it does in populations of differentiated mammalian cells, or remains constant, as it does in early sea urchin embryos. T