𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Functional PLGA Scaffolds for Chondrogenesis of Bone-Marrow-Derived Mesenchymal Stem Cells

✍ Scribed by Kwideok Park; Kyoung-Jin Cho; Jae-Jin Kim; Ik-Hwan Kim; Dong Keun Han

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
533 KB
Volume
9
Category
Article
ISSN
1616-5187

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Two chondrogenic factors, Dex and TGF‐__β__1, were incorporated into PLGA scaffolds and their chondrogenic potential was evaluated. The Dex‐loaded PLGA scaffold was grafted with AA and heparin, the heparin‐immobilized one was then reacted with TGF‐__β__1, yielding a PLGA/Dex‐TGF (PLGA/D/T) scaffold. The scaffolds were seeded with rabbit MSCs and cultured for 4 weeks. The results show that the scaffolds including chondrogenic factors strongly upregulated the expression of cartilage‐specific genes and clearly displayed type‐II collagen immunofluorescence. The functionalized PLGA scaffolds could provide an appropriate niche for chondrogenic differentiation of MSC without a constant medium supply of Dex and TGF‐__β__1.

magnified image

📜 SIMILAR VOLUMES

Chondrogenesis of human bone marrow-deri
Chondrogenesis of human bone marrow-derived mesenchymal stem cells in agarose culture
✍ Charles Huang, C.-Y. ;Reuben, Paul M. ;D'Ippolito, Gianluca ;Schiller, Paul C. ; 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 567 KB

## Abstract Mesenchymal stem cells derived from human bone marrow (hBM‐MSCs) can differentiate into chondrogenic cells for the potential treatment of injured articular cartilage. To evaluate agarose gels as a supportive material for chondrogenesis of hBM‐MSCs, this study examined chondrogenesis of

In vitro chondrogenesis of human synoviu
In vitro chondrogenesis of human synovium-derived mesenchymal stem cells: Optimal condition and comparison with bone marrow-derived cells
✍ Shinichi Shirasawa; Ichiro Sekiya; Yusuke Sakaguchi; Kazuyoshi Yagishita; Shizuk 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 611 KB

There are increasing reports that mesenchymal stem cells (MSCs) are present in various tissues other than bone marrow, including synovium. Here we investigated the optimal conditions for in vitro chondrogenesis of human synovium-derived MSCs and compared these cells with bone marrow-derived MSCs, es

Dual-Growth-Factor-Releasing PCL Scaffol
Dual-Growth-Factor-Releasing PCL Scaffolds for Chondrogenesis of Adipose-Tissue-Derived Mesenchymal Stem Cells
✍ Sung Mook Lim; Sung Hwan Jang; Se Heang Oh; Soon Hong Yuk; Gun Il Im; Jin Ho Lee 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 857 KB

## Abstract Polycaprolactone/Pluronic F127 porous scaffolds are prepared using a modified melt‐molding particulate‐leaching method. The scaffolds are highly porous (about 90% porosity) and have open‐cellular pore structures. Growth factors (TGF‐__β__~2~, BMP‐7 or dual TGF‐__β__~2~/BMP‐7) can be eas

Induction of bone marrow mesenchymal ste
Induction of bone marrow mesenchymal stem cell chondrogenesis following short-term suspension culture
✍ John D. Kisiday; Christina M. Lee; C. Wayne McIlwraith; David D. Frisbie 📂 Article 📅 2010 🏛 Elsevier Science 🌐 English ⚖ 255 KB

## Abstract The objective of this study was to evaluate mesenchymal stem cell (MSC) chondrogenesis following incubation in chondrogenic suspension cultures from which single cells were obtained. MSCs were maintained in suspension over a nonadherent surface for 3 days, dissociated into a suspension,

Cross-linking affects cellular condensat
Cross-linking affects cellular condensation and chondrogenesis in type II collagen-GAG scaffolds seeded with bone marrow-derived mesenchymal stem cells
✍ Scott M. Vickers; Tobias Gotterbarm; Myron Spector 📂 Article 📅 2010 🏛 Elsevier Science 🌐 English ⚖ 273 KB

## Abstract The formation of cartilaginous tissue by chondroprogenitor cells, whether in vivo or in vitro, appears to require a critical initial stage of “condensation” in which intercellular space is reduced through an aggregation of cells, leading to development of cell‐to‐cell junctions followed

Characterisation and developmental poten
Characterisation and developmental potential of ovine bone marrow derived mesenchymal stem cells
✍ Rosa C. McCarty; Stan Gronthos; Andrew C. Zannettino; Bruce K. Foster; Cory J. X 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 446 KB 👁 2 views

## Abstract Since discovery, significant interest has been generated in the potential application of mesenchymal stem cells or multipotential stromal cells (MSC) for tissue regeneration and repair, due to their proliferative and multipotential capabilities. Although the sheep is often used as a lar