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Functional interaction between TRP4 and CFTR in mouse aorta endothelial cells

✍ Scribed by Lin Wei; Marc Freichel; Martine Jaspers; Harry Cuppens; Jean-Jacques Cassiman; Guy Droogmans; Veit Flockerzi; Bernd Nilius

Publisher
BioMed Central
Year
2001
Tongue
English
Weight
542 KB
Volume
1
Category
Article
ISSN
1472-6793

No coin nor oath required. For personal study only.

✦ Synopsis

Background: This study describes the functional interaction between the putative Ca 2+ channel TRP4 and the cystic fibrosis transmembrane conductance regulator, CFTR, in mouse aorta endothelium (MAEC).

Results: MAEC cells express CFTR transcripts as shown by RT-PCR analysis. Application of a phosphorylating cocktail activated a Cl -current with characteristics similar to those of CFTR mediated currents in other cells types (slow activation by cAMP, absence of rectification, block by glibenclamide). The current is present in trp4 +/+ MAEC, but not in trp4 -/-cells, although the expression of CFTR seems unchanged in the trp4 deficient cells as judged from RT-PCR analysis.

Conclusions:

It is concluded that TRP4 is necessary for CFTR activation in endothelium, possibly by providing a scaffold for the formation of functional CFTR channels.

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