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Differential effects of Matrigel and its components on functional activity of CFTR and ENaC in mouse endometrial epithelial cells

โœ Scribed by Jian Zhi Yang; Alice Lok Sze Ho; Louis Chukwuemeka Ajonuma; Sun Yee Lam; Lai Ling Tsang; Ning Tang; Dewi Kenneth Rowlands; Yu Lin Gou; Yiu Wa Chung; Hsiao Chang Chan

Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
161 KB
Volume
27
Category
Article
ISSN
1065-6995

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โœฆ Synopsis

Abstract

Our previous studies have observed an effect of Matrigel, a solubilized basement membrane preparation extracted from the Engelbrethโ€”Holmโ€”Swarm (EHS) mouse sarcoma, on the expression of ion channels in mouse endometrial epithelia; namely the cystic fibrosis transmembrane conductance regulator (CFTR), a cAMPโ€dependent Cl^โˆ’^channel, and the epithelial sodium channel (ENaC). The present study further investigated the effects of Matrigel and its individual components on the functional expression of CFTR and ENaC using the shortโ€circuit current (Isc) technique. The results showed that different components of Matrigel, namely growth factors, laminin and collagen, had differential effects on the functional activity of the two ion channels in murine endometrial epithelium. The information obtained may be useful for designing future in vitro culture models to investigate the functional roles of these ion channels in the endometrium.

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