Functional genetic tests of DNA mismatch repair protein activity in Saccharomyces cerevisiae
β Scribed by Piotr Polaczek; Aaron P Putzke; Kahan Leong; Grant A Bitter
- Book ID
- 117523062
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 150 KB
- Volume
- 213
- Category
- Article
- ISSN
- 0378-1119
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The repair of double-strand gaps (DSGs) in the plasmid DNA of radiosensitive mutants of Saccharomyces cerevisiae has been analyzed. The proportion of repair events that resulted in complete plasmid DNA DSG recovery was close to 100% in Rad+ cells. Mutation rad55 does not influence the efficiency and
The survival of plasmid YRpl2 treated in vitro with ultraviolet-or 7-radiation, or with restriction endonucleases, has been used to investigate in vivo RAD gene activity in Saccharomyces cerevisiae. Yields of pyrmidine dimers or single and double strand breaks in plasmid DNA were assayed by physical