Hybrid plasmids were constructed by combining in vitro the Escherichia coli plasmid pGA22, which carries the genes determining resistance to kanamycin, tetracycline, chloramphenicol and ampicillin, with the cryptic plasmids, pCG1 and pCG2, of Corynebacterium glutamicum. The hybrid plasmids were intr
✦ LIBER ✦
Functional expression of the glutamate uptake system from Corynebacterium glutamicum in Escherichia coli
✍ Scribed by Andreas Burkovski; Reinhard Krämer
- Book ID
- 109320534
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 365 KB
- Volume
- 127
- Category
- Article
- ISSN
- 0378-1097
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## Abstract This article reports the cell‐free expression of functional Lipase B from __Candida antarctica__ (CalB) in an __Escherichia coli__ extract. Although most of the cell‐free synthesized CalB was insoluble under conventional reaction conditions, the combined use of molecular chaperones led