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Functional Conservation in the Putative Substrate Binding Site of the Sucrose Permease from Escherichia coli †

✍ Scribed by Sahin-Tóth, Miklós; Kaback, H. Ronald

Book ID
126011278
Publisher
American Chemical Society
Year
2000
Tongue
English
Weight
129 KB
Volume
39
Category
Article
ISSN
0006-2960

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✍ Jianhua Wu; Stathis Frillingos; John Voss; H. Ronald Kaback 📂 Article 📅 1994 🏛 Cold Spring Harbor Laboratory Press 🌐 English ⚖ 848 KB

## Abstract By using a lactose permease mutant containing a single Cys residue in place of Val 331 (helix X), conformational changes induced by ligand binding were studied. With right‐side‐out membrane vesicles containing Val 331 → Cys permease, lactose transport is inactivated by either __N__‐ethy