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Energy-dependent masking of substrate binding sites of the lactose permease of Escherichia coli

✍ Scribed by Martien Benard-Bentaboulet; Adam Kepes

Book ID: 115721964
Publisher: Elsevier Science
Year: 1973
Tongue: English
Weight: 727 KB
Volume: 307
Category: Article
ISSN: 0005-2736
DOI: 10.1016/0005-2736(73)90037-0

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## Abstract By using a lactose permease mutant containing a single Cys residue in place of Val 331 (helix X), conformational changes induced by ligand binding were studied. With right‐side‐out membrane vesicles containing Val 331 → Cys permease, lactose transport is inactivated by either __N__‐ethy