Functional and biochemical parameters of activation related to macrophage cytostatiic effects on tumor cells

✍ Scribed by R. Keller; Ruth Keist; R. J. Ivatt

• Publisher: John Wiley and Sons
• Year: 1974
• Tongue: French
• Weight: 619 KB
• Volume: 14
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910140515

✦ Synopsis

Abstract

The cytostatic action of non‐immune, activated peritoneal rat macrophages (AM) on tumor targets largely depends upon the duration of their culture in vitro. AM show full cytostatic activity for the first 12‐24 h of culture followed by gradual loss. This distinctive pattern of cytostatic activity displayed by AM was compared with the course of a variety of functional, metabolic and biochemical parameters. DNase, RNase, esterase, acid phosphatase, glucose oxidation and cellular adherence remained unchanged or decreased whereas prolonged culture led to an increase in activity of other lysosomal enzymes. Pinocytosis and phagocytosis were most marked in macrophages between 12‐60 h whereas elevation in macrophage protein synthesis was high only after 4 to 12 h of culture, followed by a gradual decline. Thus the capacity of macrophages to stop proliferation of tumor targets closely parallels the level of protein synthesis, whereas other parameters of macrophage function such as lysosomal enzymes, glucose oxidation, cellular adherence, pinocytosis and phagocytosis, manifested a clearly different timecourse.