Functional analysis of the finO distal region of plasmid R1
β Scribed by Monika R. Nuk; Andreas Reisner; Martina Neuwirth; Katrin Schilcher; Roland Arnold; Andre Jehl; Thomas Rattei; Ellen L. Zechner
- Book ID
- 113855478
- Publisher
- Elsevier Science
- Year
- 2011
- Tongue
- English
- Weight
- 410 KB
- Volume
- 65
- Category
- Article
- ISSN
- 0147-619X
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Lambda phages carrying the R100 finO gene have been isolated from an R100:: lambda cointegrate in which lambda was inserted into the R100 traD gene at kb coordinate 72.1. Physical analyses of these phages place the finO gene within R100 SalI fragment D, near kb coordinate 82.0. Analysis of proteins
The synthesis of the rate-limiting RepA replication initiator protein of plasmid R1 is negatively controlled by an antisense RNA, CopA. The regulation is posttranscriptional and involves an inhibitory effect on RepA translation mediated by the binding of CopA to its target (CopT) in the leader regio