Function of the LFA-1 and T4 molecules in the direct activation of resting human B lymphocytes by T lymphocytes

✍ Scribed by Thomas F. Tedder; Reinhold E. Schmidt; Christopher E. Rudd; Mary M. Kornacki; Jerome Ritz; Stuart F. Schlossman

• Publisher: John Wiley and Sons
• Year: 1986
• Tongue: English
• Weight: 576 KB
• Volume: 16
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830161212

✦ Synopsis

Function of the LFA-1 and T4 molecules in the direct activation of resting human B lymphocytes by T lymphocytes* Activated T lymphocytes can provide all of the signals necessary to induce the proliferation of resting B lymphocytes. The activation signal is presumably initiated through direct T-B lymphocyte contact. The role of the leukocyte function antigen-1 (LFA-1) and T4 molecules in the activation of purified, small B lymphocytes by mitomycin C-treated T lymphocytes was examined by using monoclonal antibodies that react with and inhibit the function of these molecules. Anti-LFA-1 antibody binding significantly inhibited T-B lymphocyte interactions that result in B lymphocyte proliferation. In contrast, the presence of anti-T4 antibodies at concentrations as high as 100 pg/ml did not inhibit this interaction. These results indicate that the B lymphocyte activation signal may not be mediated through the interaction of T4 molecules with major histocompatibility complex class I1 antigens of the B lymphocyte but is a cell-cell contact-dependent event that is facilitated by LFA-1 molecules.