We previously reported that pertussis toxin-sensitive GTP-binding protein is involved in prostaglandin F2, (PGF,,)-induced phosphoinositide (PI) hydrolysis in osteoblast-like MC3T3-EI cells [Miwa et al. (1 990): Biochem Biophys Res Commun 171 :1229-12351. In the present study, we investigated the m
Function of Ca2+ influx in phospholipase D activation induced by prostaglandin F2α in osteoblast-like cells: Involvement of tyrosine kinase
✍ Scribed by O. Kozawa; A. Suzuki; Y. Watanabe; J. Shinoda; Y. Oiso
- Book ID
- 116163650
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 436 KB
- Volume
- 52
- Category
- Article
- ISSN
- 0952-3278
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## Abstract We previously reported that prostaglandin F~2α~ (PGF~2α~) receptor is coupled to pertussis toxin (PTX)–sensitive GTP‐binding protein (G protein) in osteoblast‐like MC3T3‐E1 cells [Miwa et al. (1990): Biochem Biophys Res Commun 171:1229–1235]. In the present study, we examined the effect
In cloned osteoblast-like cells, MC3T3-EII prostaglandin F, , (PGF,,) stimulated arachidonic acid (AA) release in a dose-dependent manner in the range between 1 n M and 10 pM. 12-O-tetradecanoylphorbol-l3-acetate (TPA), a protein kinase C (PKC) activator, which by itself had little effect on AA rele