In cloned osteoblast-like cells, MC3T3-EII prostaglandin F, , (PGF,,) stimulated arachidonic acid (AA) release in a dose-dependent manner in the range between 1 n M and 10 pM. 12-O-tetradecanoylphorbol-l3-acetate (TPA), a protein kinase C (PKC) activator, which by itself had little effect on AA rele
Autoregulation of prostaglandin E2-induced Ca2+ influx in osteoblast-like cells: Inhibition by self-induced activation of protein kinase C
β Scribed by Haruhiko Tokuda; Masaichi Miwa; Yutaka Oiso; Osamu Kozawa
- Book ID
- 113388505
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 411 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0898-6568
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## Abstract We previously reported that prostaglandin F~2Ξ±~ (PGF~2Ξ±~) receptor is coupled to pertussis toxin (PTX)βsensitive GTPβbinding protein (G protein) in osteoblastβlike MC3T3βE1 cells [Miwa et al. (1990): Biochem Biophys Res Commun 171:1229β1235]. In the present study, we examined the effect
Exposure of osteoblast-like MC3T3-El cells to sodium arsenite (arsenite) increased the level of heat shock protein 27 (hsp27). The effect of arsenite was dose-dependent in the range of 50 to 200 pM. Arsenite also stimulated arachidonic acid release dose-dependently in the range between 50 and 200 pM