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Free tryptophan and indole-3-acetic acid levels in the leaves and vascular pathways ofRicinus communisL.

✍ Scribed by J. R. F. Allen; D. A. Baker


Publisher
Springer-Verlag
Year
1980
Tongue
English
Weight
505 KB
Volume
148
Category
Article
ISSN
0032-0935

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✦ Synopsis


Levels of free tryptophan in the leaves, phloem and xylem saps of Ricinus communis L. were determined by colorimetric assay. Values of 0~38 gg ml-1 in root pressure sap and 96.0 pg ml ~ in phloem sap were recorded. Tryptophan levels were highest in mature and senescing leaves. Levels of indoleacetic acid (IAA) in the phloem sap and leaves were determined by gas chromatography-mass spectrometry using a deuterated internal standard. A mean value of 13.0 ng ml-1 was recorded in phloem sap. The distribution in the leaves showed an inverse relationship to that of tryptophan, being highest in young leaves.


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Synthesis of [13C]-isotopomers of indole
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## Abstract The direct conversion of indole to indole‐3‐acetic acid without tryptophan as an intermediate has previously been shown to occur __in vivo__, as well as __in vitro__, with seedlings of plants. In order to facilitate the purification of the enzymes that carry out the enzymatic synthesis