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Free Nonimmobilized Ligands as a Tool for Purification of Proteins

✍ Scribed by Patchornik, Guy; Albeck, Amnon


Book ID
127246114
Publisher
American Chemical Society
Year
2005
Tongue
English
Weight
233 KB
Volume
16
Category
Article
ISSN
1043-1802

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Restriction sites were introduced into the Eschcrichia coli melR gene that facilitated the fusion of other proteins to MelR. Both R-galactosidase and the constant domain of the human IgG kappa light chain were fused to MelR. However, whilst unmodified MelR could be over-expressed, neither MelR fusio