Free cyclic AMP increases in PC12 cells on depolarization

✍ Scribed by Naveen Agnihotri; William S. Kisaalita; Charles H. Keith

• Book ID: 101244425
• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 125 KB
• Volume: 47
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/(sici)1097-4547(19970301)47:5<555::aid-jnr11>3.0.co;2-x

✦ Synopsis

The temporal dynamics of the intracellular second messenger cyclic AMP (cAMP) were monitored in living PC12 cells by digital fluorescence ratio imaging using FlCRhR, a single-excitation dual-emission cAMP indicator. When the cells were depolarized by exposure to high K 1 , the free cAMP concentration was elevated, and then slowly decreased back to resting levels when the depolarizing stimulus was removed. Furthermore, the cAMP elevation due to depolarization decreased with successive depolarizations. J.