Free cyclic AMP increases in PC12 cells
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Naveen Agnihotri; William S. Kisaalita; Charles H. Keith
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Article
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1997
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John Wiley and Sons
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English
โ 125 KB
The temporal dynamics of the intracellular second messenger cyclic AMP (cAMP) were monitored in living PC12 cells by digital fluorescence ratio imaging using FlCRhR, a single-excitation dual-emission cAMP indicator. When the cells were depolarized by exposure to high K 1 , the free cAMP concentratio