Free calcium in Xenopus embryos measured with ion-selective microelectrodes
β Scribed by Rink, T. J.; Tsien, R. Y.; Warner, A. E.
- Book ID
- 109717597
- Publisher
- Nature Publishing Group
- Year
- 1980
- Tongue
- English
- Weight
- 398 KB
- Volume
- 283
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/283658a0
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π SIMILAR VOLUMES
Ca2+ ion activities and pH were measured in the odontoblast/predentin region of rat incisors by means of the microelectrode technique. In Ringer solution, the apparent resting membrane potential of odontoblasts was determined to be -24 +/- 4 mV (mean +/- SE), whereas the odontoblast intracellular pH
Potassium ionselective microelectrodes with a tip diameter of 10 pm are described. The ionophore used is 2,2'-bie[3,4-(16-mow-6)-2-nitrophenylc~b~ox~ethyl]~~decane in a conventional mixture with sodium tetraphenylborate, an ether and PVC. The electrode provides Nernstian response over the range 1O-5