In vivo measurements with a potassium ion-selective microelectrode based on a new bis(crown ether)

✍ Scribed by J. Tarcali; G. Nagy; K. Tóth; E. Pungor; G. Juhász; T. Kukorelli

• Book ID: 104100541
• Publisher: Elsevier Science
• Year: 1985
• Tongue: English
• Weight: 417 KB
• Volume: 178
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/s0003-2670(00)86273-x

✦ Synopsis

Potassium ionselective microelectrodes with a tip diameter of 10 pm are described. The ionophore used is 2,2'-bie[3,4-(16-mow-6)-2-nitrophenylc~b~ox~ethyl]~~decane in a conventional mixture with sodium tetraphenylborate, an ether and PVC. The electrode provides Nernstian response over the range 1O-5-lO-1 M potassium activity. Its selectivity is shown to be similar to that of a valinomycin-based microelectrode. After the electrode has aged for 24 h, the 96% response tie at the lo-' M potassium ion level is 2.0 * 0.6 s. The applicability of the bis(crown ether) electrode for measurements in vivo is proved by monitoring the changes in potassium activity in different areas of the brain of anaesthetized rats after administration of veratrine.