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Fractionation of poly (dA-dT) by gel chromatography

✍ Scribed by Choong-Gook Jang; Paul Bartl

Publisher
Wiley (John Wiley & Sons)
Year
1971
Tongue
English
Weight
449 KB
Volume
10
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

A commercial sample of poly (dA‐dT), a copolymer of 2′‐deoxyribosyladenosine (dA) and 2′‐deoxyribosylthymidine (dT) of perfectly alternating sequence, was fractionated by chromatography on Agarose gel. Paucidisperse fractions of different molecular size were obtained. The plot of log s^0^~20,w~ values shows a linear dependence on V~e~. The buoyant densities of individual fractions do not differ over the molecular size range studied. On the other hand, the heat‐induced hyperchromic effect was found to depend on molecular size below a certain limit, s^0^~20,w~ = 4.12 S.

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