Formation of microparticulate protein powder using a supercritical fluid antisolvent

✍ Scribed by Sang-Do Yeo; Gio-Bin Lim; Pablo G. Debendetti; Howard Bernstein

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 590 KB
• Volume: 41
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260410308

✦ Synopsis

Gas antisolvent (GAS) expansion of dirnethylsulfoxide (DMSO) and N,N-dimethylformamide (DMFA) solutions with supercritical carbon dioxide was used to produce biologically active powders of insulin. Powders with 90% of the particles smaller than 4 prn and 10% smaller than 1 pm were obtained under all conditions tested when the process was operated continuously, with small liquid droplets sprayed into a flowing supercritical continuum. Slow pressurization of the stagnant protein solution resulted in larger particles. In vivo tests on rats revealed no difference between the biological activity of processed and unprocessed insulin. GAS processing of organic solutions appears to be a reliable and effective method for the production of dry, biologically active microparticulate powders of peptides and proteins.