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Formation of acetyl-CoA inZymomonas mobilisby a pyruvate dehydrogenase complex

โœ Scribed by Stephanie Bringer-Meyer; Hermann Sahm


Publisher
Springer
Year
1993
Tongue
English
Weight
309 KB
Volume
159
Category
Article
ISSN
0302-8933

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A radiochemical method for assaying pyruvate dehydrogenase complex and acetyl-coenzyme A synthetase is described, using [2-'%]pyruvate and [ 1-r4C]acetate, respectively, as radiolabeled precursors. The assay is based on nonenzymatic O-acylation of excess dithioerythritol (DTE) by enzymatically forme