A new radiochemical method for determina
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Bodo Liedvogel
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Article
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1985
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Elsevier Science
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English
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A radiochemical method for assaying pyruvate dehydrogenase complex and acetyl-coenzyme A synthetase is described, using [2-'%]pyruvate and [ 1-r4C]acetate, respectively, as radiolabeled precursors. The assay is based on nonenzymatic O-acylation of excess dithioerythritol (DTE) by enzymatically forme