A new radiochemical method for determination of pyruvate dehydrogenase complex and acetyl-coenzyme a synthetase

✍ Scribed by Bodo Liedvogel

• Publisher: Elsevier Science
• Year: 1985
• Tongue: English
• Weight: 637 KB
• Volume: 148
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(85)90643-8

✦ Synopsis

A radiochemical method for assaying pyruvate dehydrogenase complex and acetyl-coenzyme A synthetase is described, using [2-'%]pyruvate and [ 1-r4C]acetate, respectively, as radiolabeled precursors. The assay is based on nonenzymatic O-acylation of excess dithioerythritol (DTE) by enzymatically formed acetyl-coenzyme A. [ I-'4C]Acetyl-DTE is easily extracted from the incubation mixture by organic solvents and separated from the unreacted labeled substrates.