𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Formation and regeneration of protoplasts from Gibberella fujikuroi

✍ Scribed by Dr. B. Brückner; R. May; B. Loeffler

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
431 KB
Volume
30
Category
Article
ISSN
0233-111X

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✦ Synopsis

The preparation and regeneration of protoplasts from Gibberella .fujikuroi was examined. High yields of protoplasts were obtained by using a mixture of lyophilized enzyme preparation from the snail Helix pomaria and a laboratory prepared mycolytic enzyme system from Trichodernia harzianurn. The physiological stage of the mycelium and the mycelium amount were found to be important factors in the determination of the protoplast yield. Regeneration could be achieved on the surface of culture media osmotically stabilized with NaCI. KCI, mannitol or sorbitol.

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