𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Activity of mutagens in the fungus Gibberella fujikuroi

✍ Scribed by Dr. R.; B. Brückner; D. Blechschmidt; W. Fischer

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
143 KB
Volume
30
Category
Article
ISSN
0233-111X

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✦ Synopsis

The efficacy of a number of mutagens was cxamined for Gibberella fujikuroi. Nitrosogudnidine (MNNG) was the most effective mutagen inducing mainly morphological and pigment mutants. X-ray produced small mutagenic effect. After UV-irradiation pigment mutants developed for the most part and a combination of UV-and A'-ray-irradiation mainly influenced the growth rate. A combination of UV-irradiation with M N N G elicited the highest number of mutants with increased GA, production.

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Transformation of the filamentous fungus Gibberella fujikuroi using the Aspergillus niger niaD gene encoding nitrate reductase
✍ Sánchez-Fernández, Rocio ;Unkles, Shiela E. ;Campbell, Edward I. ;Macro, Janet A 📂 Article 📅 1991 🏛 Springer 🌐 English ⚖ 323 KB

A transformation system for Gibberella fujikuroi based on the Aspergillus niger nitrate reductase gene (niaD) was developed. A strain (designated SG140) carrying a non-reverting niaD mutation (niaD11) was generated by screening mutagenised cells for non-growth on nitrate as sole nitrogen source. Tra