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Fluorometric measurement of DNA reassociation kinetics

✍ Scribed by K.P. Hanson; N.B. Zvonareva; Y.Ya. Ostashevsky; B.D. Zhivotovsky

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
209 KB
Volume
94
Category
Article
ISSN
0003-2697

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✦ Synopsis

A simple, highly sensitive method for assessment of the kinetics of reassociation of DNA has been developed. The method is based on the difference between the intensity of fluorescence of the ethidium bromide complex with single-and double-stranded DNA and requires no preliminary preparative separation of the molecules.

Fluorometric estimation of DNA reassociation. Ethidium bromide (Serva) in 0.14 M

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Rodent DNA reassociation kinetics
Rodent DNA reassociation kinetics
✍ Lourdes Santiago; Adrian V. Rake πŸ“‚ Article πŸ“… 1973 πŸ› Springer 🌐 English βš– 425 KB

The kinetics of reassociation of the nuclear DNA of eight rodents has been examined to determine the amount and repetition frequency of various DNA fractions. Each rodent had at least two distinct, repeated DNA fractions, one group repeated about 300 times and the other repeated about 50,000 times.