Comparative DNA reassociation kinetics of cranes
β Scribed by Carey Krajewski
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 50 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0006-2928
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The kinetics of reassociation of the nuclear DNA of eight rodents has been examined to determine the amount and repetition frequency of various DNA fractions. Each rodent had at least two distinct, repeated DNA fractions, one group repeated about 300 times and the other repeated about 50,000 times.
A simple, highly sensitive method for assessment of the kinetics of reassociation of DNA has been developed. The method is based on the difference between the intensity of fluorescence of the ethidium bromide complex with single-and double-stranded DNA and requires no preliminary preparative separat