Fluorometric determination of the DNA content of cells cultured in tissue culture plates
โ Scribed by Margarete Goppelt-Struebe; Marina Golombek
- Book ID
- 118902558
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 189 KB
- Volume
- 151
- Category
- Article
- ISSN
- 0022-1759
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A simple, reproducible fluorescent assay has been described which can be used confidently at microgram and submicrogram levels. The method is most suitable for cell cycle and cell growth analysis using [3H]thymidine as the radioactive tracer. A modified method is also described for preparing diamino
A simple assay is described using bisbenzimidazole (Hoechst 33258) to determine cellular DNA content in 96-well tissue cultures plates. At time points of interest, the plates are emptied of media and stored frozen. When the assay is to be performed, cultures are briefly incubated in distilled water