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Fluorometric determination of spermidine using o-phthalaldehyde: Optimum reaction conditions and tests of identity

✍ Scribed by Rolf Håkanson; Anna-Lisa Rönnberg


Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
495 KB
Volume
54
Category
Article
ISSN
0003-2697

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The use of the o-phthalaldehyde reaction
✍ Stanley A. Robrish; Christopher Kemp; William H. Bowen 📂 Article 📅 1978 🏛 Elsevier Science 🌐 English ⚖ 618 KB

Alkaline hydrolysis of protein followed by reaction with o-phthalaldehyde has permitted the determination of less than 10 ng of protein fluorometrically. When intact proteins were analyzed with o-phthalaldehyde the reaction was less sensitive. This reaction has been applied to analysis of the protei