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Fluorometric determination of phosphatidylcholine as a measure of phospholipid methylation

✍ Scribed by D.M. Dandurand; F.L. Kiechle; D.R. Strandbergh; B. Zak; J.D. Artiss

Publisher: Elsevier Science
Year: 1991
Tongue: English
Weight: 500 KB
Volume: 196
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(91)90478-c

No coin nor oath required. For personal study only.

✦ Synopsis

The successive methylation of phosphatidylethanolamine to phosphatidylcholine (phospholipid methylation) has been measured by the incorporation of S-[methyl-3H]adenosylmethionine or colorimetric assay of phosphatidylcholine extracted from adipocyte plasma membranes. A fluorometric assay for phosphatidylcholine was developed to measure phospholipid methylation. This assay is 10 times more sensitive than the colorimetric assay and demonstrates no significant interference with other methylated phospholipids. The fluorometric assay was used to determine a biphasic insulin dose response in adipocyte plasma membranes. This fluorometric assay for phosphatidylcholine represents an alternative method for monitoring phospholipid methylation, especially when increased sensitivity is required.

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