## Fluorimetric determination of some aliphatic and cyclic amines with 3-carboxy-7-hydroxycoumarin It was observed in this laboratory that the reaction of organic aliphatic and cyclic amines with 3-carboxy-7-hydroxycoumarin resultecl in an increase in fluorescence of the reaction mixture. This inc
Fluorometric determination of amphetamines with 3-carboxy-7-hydroxycoumarin
β Scribed by J. T. Stewart; D. M. Lotti
- Publisher
- John Wiley and Sons
- Year
- 1971
- Tongue
- English
- Weight
- 356 KB
- Volume
- 60
- Category
- Article
- ISSN
- 0022-3549
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A specific and simple fluorometric method for the determination of serum arginine was developed which consists of (1) deproteinization of serum sample with 10% perchloric acid, (2) blocking thiol compounds with N-ethylmaleimide, (3) fluorescence reaction with 2,3-naphthalenedicarbaldehyde in 0.2 M b
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From the above graph it will be seen that readings can be made over the whole range between IO and 200 pg. After 200 pg, however, the graph flattens out and is, therefore, of little USC for sensitive work. For practical purposes in order to I~eJc~orccs p. 4Ss'.