𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Fluorometric assay for urea in urine, plasma, and tubular fluid

✍ Scribed by Richard J. Roman; Joseph V. Bonventre; Claude P. Lechene

Book ID
102985428
Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
501 KB
Volume
98
Category
Article
ISSN
0003-2697

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✦ Synopsis

Simple and sensitive two-step fluorometric assays for urea in microliter and nanoliter samples of biologic fluid are presented. Urea was reacted with urease to release ammonia. The ammonia produced was combined with a pthalaldehyde-mercaptoethanol reagent at pH 7.4 to produce a stable fluorescent product. The fluorescence of unknowns and urea standards were measured using a filter fluorometer and compared. Fluorescent intensity of the reaction product varied linearly with urea concentration over a wide range (O-50 mM) for both microliter and nanoliter samples. Recovery of urea when added to urine or plasma was complete.

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