Fluorometric assay for urea in urine, pl
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Richard J. Roman; Joseph V. Bonventre; Claude P. Lechene
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Article
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1979
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Elsevier Science
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English
β 501 KB
Simple and sensitive two-step fluorometric assays for urea in microliter and nanoliter samples of biologic fluid are presented. Urea was reacted with urease to release ammonia. The ammonia produced was combined with a pthalaldehyde-mercaptoethanol reagent at pH 7.4 to produce a stable fluorescent pr