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Fluorimetric DNA assay for cell growth estimation

โœ Scribed by Jagdish Rao; William R. Otto

Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
707 KB
Volume
207
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A growth assay using the fluorescent dye Hoechst 33258 has been developed which allows sensitive and rapid analysis of the DNA content of a variety of cell types including keratinocytes and mucus-secreting cells, and which requires a minimum of liquid handling. The assay can detect as few as 500 diploid human cells, and is compatible with the simultaneous detection of [3H]thymidine incorporation in the same cultures.

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โœ R. Sreekumaran Nair; R. Paulmurugan; A. J. A. Ranjit Singh ๐Ÿ“‚ Article ๐Ÿ“… 2002 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 150 KB

## Abstract The intactness of DNA is an important part of the normal cellular structure. Any change to the DNA in the form of breaks leads to a change in the integrity, which in turn leads to abnormality in the cellular activity. Many discrepancies have been reported among the various methods of de