A rapid colorimetric assay for the estimation of microgram quantities of DNA
โ Scribed by David V. Gold; Dan Shochat
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 336 KB
- Volume
- 105
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
## Abstract The intactness of DNA is an important part of the normal cellular structure. Any change to the DNA in the form of breaks leads to a change in the integrity, which in turn leads to abnormality in the cellular activity. Many discrepancies have been reported among the various methods of de
The use of the cationic thiazine dye toluidine blue for solution measurements of DNA in the submicrogram range was studied with particular regard to the measurement of the small quantities of DNA synthesized in a typical polymerase chain reaction (PCR). Tolouidine blue shows a decrease in absorption