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Fluorimetric determination of nucleic acid using the enhancement of terbium–gadolinium–nucleic acid–cetylpyridine bromide system

✍ Scribed by Lei Li; Jinghe Yang; Xia Wu; Changxia Sun; Guangjun Zhou

Book ID
117223233
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
234 KB
Volume
59
Category
Article
ISSN
0039-9140

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A dimeric derivative of ethidium is used for fluorimetric assay of nucleic acids. Due to the very high binding affinity of this derivative for DNA and RNA, a significant increase of sensitivity of the ethidium fluorimetric technique for nucleic acids determination is obtained. Using a commercially a