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Fluorescent labeling of the surface proteins of erythrocyte membranes using cycloheptaamylose-fluorescamine complex

โœ Scribed by Kazuyasu Nakaya; Masayo Yabuta; Fumio Iinuma; Toshio Kinoshita; Yasuharu Nakamura

Book ID
118310440
Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
711 KB
Volume
67
Category
Article
ISSN
0006-291X

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A protein assay is described in which the sample is spotted on a Sartorius membrane filter and dansylated with cycloheptaamylose-dansyl chloride complex dissolved in aqueous urea solution. The fluorescence intensity of the spot on the membrane was directly measured by a scanning fluorometer. This as