Two general methods for labeling synthetic peptides with a 5-dimethylamino-1-napthalenesulfonyl idansyl) group at the C-terminal residue using solid phase peptide synthesis (SPPS) are described. Dansylated peptides are ideal substrates for fluorometric proteolytic enzyme assays.
Fluorescent labeling of peptides on solid phase
β Scribed by Katritzky, Alan R.; Yoshioka, Megumi; Narindoshvili, Tamari; Chung, Alfred; Johnson, Jodie V.
- Book ID
- 118277620
- Publisher
- Royal Society of Chemistry
- Year
- 2008
- Tongue
- English
- Weight
- 194 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1477-0520
- DOI
- 10.1039/b811693h
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## Abstract Fluorescent cell analytical techniques require the incorporation of a fluorophore into the target molecule without causing a significant change in the native conformation. Many short peptides have a limited number of reactive groups that can be labeled without affecting the biological a
amide gel was prepared according to Laemmli (4). Sample buffer containing 0.0625 M Tris-HCl (pH 6.8), 2% (w/v) SDS, 10% (v/v) glycerol, 5% (v/v) 2-mercaptoethanol, and 0.001% (w/v) bromophenol blue was loaded into the wells of the gel and allowed to stand for 5 min. Subsequently, electrophoresis was