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Fluorescence resonance energy transfer between fluorescent proteins as powerful toolkits for in vivo studies

✍ Scribed by A.L. Rusanov; A.P. Savitsky

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
228 KB
Volume
8
Category
Article
ISSN
1612-2011

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✦ Synopsis

To expand the field of research in biological systems development of extra-sensitive analytical methods is highly desirable. In this review, the latest advances in technologies relying on the fluorescence resonance energy transfer between fluorescent proteins (FP's) to visualize numerous molecular processes in living cells are discussed. Variety of FP's as well as of novel experimental techniques allows one to choose the most appropriate tools to attack concrete problems. Donor Acceptor Protease Site FRET FRET Cleavage of the linker between two fluorescent proteins by sitespecific protease resulted in essential decrease of FRET-level

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