A Method to Measure the Interaction of R
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Debbie L. Graham; Peter N. Lowe; Peter A. Chalk
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Article
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2001
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Elsevier Science
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English
⚖ 109 KB
Enhanced blue fluorescent protein (EBFP) and enhanced green fluorescent protein (EGFP) mutants of GFP in close proximity to one another can act as a fluorescence resonance energy transfer (FRET) pair. Unstructured amino acid linkers of varying length were inserted between EBFP and EGFP, revealing th