Fluorescence ?reading-out? of the molecular-recognition process
β Scribed by Aoki, Izuo; Harada, Takaaki; Sakaki, Toru; Kawahara, Yohko; Shinkai, Seiji
- Book ID
- 127329471
- Publisher
- The Royal Society of Chemistry
- Year
- 1992
- Tongue
- English
- Weight
- 424 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0022-4936
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## Abstract Direct monitoring of recognition processes at the molecular level is a valuable tool for studying reaction kinetics to assess affinity constants (e.g. drugs to receptors) and for designing rapid single step immunoassays. Methods currently used to gain information about binding processes
Edited By Margaret J. Snowling And Charles Hulme. Includes Bibliographical References And Indexes.
Fluorescence correlation spectroscopy (FCS) is a fluorescence microscopy technique that allows the study of molecular interactions in extremely low volumes, at nanomolar concentrations, even when binding is not accompanied by a fluorescence change. It can be applied directly in living cells. FCS cle