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Fluorescence of proflavine–DNA complexes: Heterogeneity of binding sites

✍ Scribed by J. C. Thomes; G. Weill; M. Daune

Publisher
Wiley (John Wiley & Sons)
Year
1969
Tongue
English
Weight
634 KB
Volume
8
Category
Article
ISSN
0006-3525

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✦ Synopsis

Measurements of the relative quantum yield of fluorescence of proflavine bound to DNA as a function of the number of bound dyes per nucleotide and the ionic strength allow the determination of the binding constants and respective number of the two types of sites previously postulated. It is demonstrated that 2-3% of the base pairs form sites where the dye is strongly bound and fluoresces normally while in the other set of sites the binding constant is 3-4 times weaker and the fluorescence completely quenched.

Comparison with complexes of Pro with double stranded polynucleotides poly (A + U), poly (I + C), poly (G + C), confirm that the strong binding sites correspond to A-T-rich regions of the I>XA while the quenched sites seem to require the presence of a neighboring guanine. The role of charge transfer in quenching of fluorescence and mutagnic act,ion is considered. An original method for the determination of free dye and bound dye, based upon the use of an external quencher is described in the Appendix.

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